Nucleosomes and centromeric DNA packaging

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Nucleosomes and centromeric DNA packaging.

The eukaryotic chromosome is a conserved structure, with the DNA double-helix wrapping around octamers of histone proteins to form the chromatin, which is further packaged into chromosomes. The centromere defines the kinetochore, the region of spindle microtubule attachment that pulls the two replicated chromatids of each chromosome apart during cell division (Fig. 1), leading to fidelity in tr...

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Centromeres of higher eukaryotes are epigenetically maintained; however, the mechanism that underlies centromere inheritance is unknown. Centromere identity and inheritance require the assembly of nucleosomes containing the CenH3 histone variant in place of canonical H3. Although H3 nucleosomes wrap DNA in a left-handed manner and induce negative supercoils, we show here that CenH3 nucleosomes ...

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Structure, dynamics, and evolution of centromeric nucleosomes.

Centromeres are defining features of eukaryotic chromosomes, providing sites of attachment for segregation during mitosis and meiosis. The fundamental unit of centromere structure is the centromeric nucleosome, which differs from the conventional nucleosome by the presence of a centromere-specific histone variant (CenH3) in place of canonical H3. We have shown that the CenH3 nucleosome core fou...

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Every chromosome needs a centromere for proper segregation during cell division. Centromeric chromatin wraps around histones, providing an anchor for kinetochore proteins and spindle attachment. It is clear why cells need centromeres, but how they form and what they look like is less so. Recent reports extend our understanding of chaperones involved in centromere formation. And other accounts o...

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ژورنال

عنوان ژورنال: Proceedings of the National Academy of Sciences

سال: 2013

ISSN: 0027-8424,1091-6490

DOI: 10.1073/pnas.1319945110